Table 1.
List of allergens contained on the MeDALL allergen-chip. Allergen-sources, allergen-names according to IUIS-standard, purification from extracts (natural allergens) or production in expression systems (recombinant allergens), glycosylation and allocation to different allergen-families are shown. Proteins that were provided by collaborating groups are highlighted by coloured boxes (column “Allergen”). For wheat allergens, # indicates the number of the respective clones. Some allergen families represented on the chip by several allergen molecules are highlighted by coloured boxes (column “Function of the protein/Allergen family”). In the separate part of the list, allergens that replaced proteins from the first variant of the microarray (Proteins No. 164–176) are listed.
| Version 1 of the MeDALL-Chip | ||||||
|---|---|---|---|---|---|---|
| No. | Allergen-source | Allergen | Rec. or natural |
CCD | Route of exposure |
Function of the protein/Allergen family |
| 1 | Birch | Bet v 1 | R | inh | PR-10 | |
| 2 | Bet v 2 | R | inh | Profilin | ||
| 3 | Bet v 4 | R | inh | Polcalcin | ||
| 4 | Alder | Aln g 1 | R | inh | PR-10 | |
| 5 | Olive | Ole e 1 | R | inh | Ole e 1-related protein | |
| 6 | Ole e 7 | N | inh | nsLTP, type 1 | ||
| 7 | Ole e 9 | R | inh | Glucanase | ||
| 8 | Japanese cedar | Cry j 1 | N | CCD | inh | Pectate lyase |
| 9 | Cypress | Cup a 1 | N | CCD | inh | Pectate lyase |
| 10 | Plane tree | Pla a 1 | R | inh | Invertase Inhibitor | |
| 11 | Pla a 2 | N | CCD | inh | Polygalacturonases | |
| 12 | Pla a 3 | R | inh | nsLTP, type 1 | ||
| 13 | Timothy grass | Phl p 1 | R | inh | Grass group 1 (Beta-Expansin) | |
| 14 | Phl p 2 | R | inh | Grass group 2/3 | ||
| 15 | Phl p 4 | N | CCD | inh | Grass group 4 (Berberine bridge enzyme) | |
| 16 | Phl p 5b | R | inh | Grass group 5 | ||
| 17 | Phl p 6 | R | inh | Grass group 5/6 | ||
| 18 | Phl p 7 | R | inh | Calcium-binding proteins (Polcalcin) | ||
| 19 | Phl p 11 | R | inh | Ole e 1-related protein | ||
| 20 | Phl p 12 | R | inh | Profilin | ||
| 21 | Bermuda grass | Cyn d 1 | N | CCD | inh | Grass group 1 (Beta-Expansin) |
| 22 | Ragweed | Amb a 1 | N | inh | Pectate lyase | |
| 23 | Mugwort | Art v 1 | N | inh | Defensin-like protein | |
| 24 | Art v 3 | N | inh | nsLTP, type 1 | ||
| 25 | Goosefoot | Che a 1 | R | inh | Ole e 1-related protein | |
| 26 | Annual mercury | Mer a 1 | R | inh | Profilin | |
| 27 | Plantain | Pla l 1 | R | inh | Ole e 1-related protein | |
| 28 | Wall pellitory | Par j 2 | R | inh | nsLTP, type 1 | |
| 29 | Saltwort | Sal k 1 | N | inh | Pectin methylesterase | |
| 30 | Latex | Hev b 1 | R | inh, skin | Rubber elongation factor | |
| 31 | Hev b 3 | R | inh, skin | Small rubber particle protein | ||
| 32 | Hev b 5 | R | inh, skin | Acidic protein | ||
| 33 | Hev b 6.01 | R | inh, skin | Hevein (Prohevein) | ||
| 34 | Hev b 8 | R | inh, skin | Profilin | ||
| 35 | Alternaria | Alt a 1 | R | inh | Acidic glycoprotein | |
| 36 | Alt a 6 | R | inh | Enolase | ||
| 37 | Aspergillus | Asp f 1 | R | inh | Mitogillin family (Ribonuclease) | |
| 38 | Asp f 3 | R | inh | Peroxysomal protein | ||
| 39 | Asp f 6 | R | inh | Mn superoxide dismutase | ||
| 40 | Cladosporium | Cla h 8 | R | inh | Mannitol dehydrogenase | |
| 41 | House dust mite | Der p 1 | N | inh | Group 1 mite allergens (Cysteine protease) | |
| 42 | Der f 1 | N | inh | Group 1 mite allergens (Cysteine protease) | ||
| 43 | Der p 2 | R | inh | Group 2 mite allergens (NPC2 family) | ||
| 44 | Der f 2 | R | inh | Group 2 mite allergens (NPC2 family) | ||
| 45 | Der p 4 | R | inh | Group 4 mite allergens (Alpha-amylase) | ||
| 46 | Der p 5 | R | inh | Group 5 mite allergens | ||
| 47 | Der p 7 | R | inh | Group 7 mite allergens | ||
| 48 | Der p 10 | R | inh | Group 10 mite allergens (Tropomyosin) | ||
| 49 | Der p 11 | R | inh | Group 11 mite allergens (Paramyosin) | ||
| 50 | Der p 14 | R | inh | Vitellogenin (Apolipophorins) | ||
| 51 | Der p 15 | R | inh | Chitin-binding domain | ||
| 52 | Der p 18 | R | inh | Chitin-binding domain | ||
| 53 | Der p 21 | R | inh | Group 21 mite allergens | ||
| 54 | Der p 23 | R | inh | Chitin-binding domain | ||
| 55 | clone 16 | R | inh | Chitin-binding domain | ||
| 56 | Storage mite | Lep d 2 | R | inh | Group 2 mite allergens (NPC2 family) | |
| 57 | Blomia tropicalis | Blo t 5 | R | inh | Group 5 mite allergens | |
| 58 | Cockroach | Bla g 1 | R | inh | Cockroach group 1 | |
| 59 | Bla g 2 | R | inh | Aspartic protease | ||
| 60 | Bla g 5 | R | inh | Glutathione S-transferase | ||
| 61 | Bla g 7 | N | inh | Tropomyosin | ||
| 62 | Anisakis | Ani s 1 | R | f | Serine protease inhibitor | |
| 63 | Ani s 3 | R | f | Tropomyosin | ||
| 64 | Cat | Fel d 1 | R | inh | Uteroglobin | |
| 65 | Fel d 2 | N | inh | Serum Albumin | ||
| 66 | Fel d 4 | R | inh | Lipocalin | ||
| 67 | Dog | Can f 1 | R | inh | Lipocalin | |
| 68 | Can f 2 | R | inh | Lipocalin | ||
| 69 | Can f 3 | N | inh | Serum Albumin | ||
| 70 | Can f 4 | R | inh | Lipocalin (Odorant-binding protein) | ||
| 71 | Can f 5 | R | inh | Arginine Esterase (Trypsin-like serine protease) | ||
| 72 | Can f 5 | R | inh | Arginine Esterase (Trypsin-like serine protease) | ||
| 73 | Can f 6 | R | inh | Lipocalin | ||
| 74 | Horse | Equ c 1 | R | inh | Lipocalin | |
| 75 | Equ c 3 | N | inh | Serum Albumin | ||
| 76 | Mouse | Mus m 1 | N | inh | Lipocalin | |
| 77 | Peanut | Ara h 1 | N | f | Storage protein, 7S globulin (Vicilins) | |
| 78 | Ara h 1 | R | f | Storage protein, 7S globulin (Vicilins) | ||
| 79 | Ara h 2 | R | f | Storage protein, 2S albumin | ||
| 80 | Ara h 3 | N | f | Storage protein, 11S globulin (Legumins) | ||
| 81 | Ara h 3 | R | f | Storage protein, 11S globulin (Legumins) | ||
| 82 | Ara h 6 | N | f | Storage protein, 2S albumin | ||
| 83 | Ara h 6 | N | f | Storage protein, 2S albumin | ||
| 84 | Ara h 8 | R | f | PR-10 | ||
| 85 | Ara h 9 | R | f | nsLTP, type 1 | ||
| 86 | Hazelnut | Cor a 1.0401 | R | f, inh | PR-10 | |
| 87 | Cor a 8 | R | f | nsLTP, type 1 | ||
| 88 | Cor a 9 | N | f | Storage protein, 11S globulin (Legumins) | ||
| 89 | Walnut | Jug r 1 | R | f | Storage protein, 2S albumin | |
| 90 | Jug r 2 | N | CCD | f | Storage protein, 7S globulin (Vicilins) | |
| 91 | Jug r 3 | R | f | nsLTP, type 1 | ||
| 92 | Almond | Pru du 3 | R | f | nsLTP, type 1 | |
| 93 | Pru du 4 | R | f | Profilin | ||
| 94 | Pru du 6 | N | f | Storage protein, 11S globulin (Legumins) | ||
| 95 | Pru du 6.01 | R | f | Storage protein, 11S globulin (Legumins) | ||
| 96 | Pru du 6.02 | R | f | Storage protein, 11S globulin (Legumins) | ||
| 97 | Cashew nut | Ana o 1 | R | f | Storage protein, 7S globulin (Vicilins) | |
| 98 | Ana o 2 | R | f | Storage protein, 11S globulin (Legumins) | ||
| 99 | Ana o 2 | N | f | Storage protein, 11S globulin (Legumins) | ||
| 100 | Ana o 2 | R | f | Storage protein, 11S globulin (Legumins) | ||
| 101 | Ana o 3 | R | f | Storage protein, 2S albumin | ||
| 102 | Pistachio | Pis v 3 | R | f | Storage protein, 7S globulin (Vicilins) | |
| 103 | Brazil nut | Ber e 1 | R | f | Storage protein, 2S albumin | |
| 104 | Sesame | Ses i 1 | N | f | Storage protein, 2S albumin | |
| 105 | Soy | Gly m 4 | R | f | PR-10 | |
| 106 | Gly m 5 | N | f | Storage protein, 7S globulin (Vicilins) | ||
| 107 | Gly m 6 | N | f | Storage protein, 11S globulin (Legumins) | ||
| 108 | Wheat | Tri a 14 | R | f, inh | nsLTP, type 1 | |
| 109 | Tri a 14 | R | f, inh | nsLTP, type 1 | ||
| 110 | Tri a 19.0101 | R | f | Storage protein, Omega 5 gliadin | ||
| 111 | Tri a aA TI | N | f, inh | Alpha-Amylase / Trypsin Inhibitors | ||
| 112 | m43 | R | f | Fragment of HMW Glutenin Bx7 | ||
| 113 | m82 | R | f | Fragment of HMW Glutenin Bx7 | ||
| 114 | Tri a 36 (191-369) | R | f, inh | Fragment of LMW Glutenin | ||
| 115 | Tri a 36 | R | f, inh | LMW Glutenin | ||
| 116 | Tri a 37 | R | f | Thionins (Alpha-purothionin) | ||
| 117 | Tri a 39 (#10) | R | inh | Serine protease inhibitors | ||
| 118 | Tri a Trx (#37) | R | inh | Oxidoreductases (Thioredoxin) | ||
| 119 | Tri a GST (#38) | R | inh | Glutathione S-transferases | ||
| 120 | Tri a 32 (#112) | R | inh | Peroxiredoxins (1-Cys-peroxiredoxin) | ||
| 121 | Tri a 12 (#123) | R | f, inh | Profilin | ||
| 122 | Tri a DH (#126) | R | inh | Dehydrins | ||
| 123 | Buckwheat | Fag e 2 | N | f | Storage protein, 2S albumin | |
| 124 | Kiwi | Act d 1 | N | f | Cysteine protease | |
| 125 | Act d 2 | N | f | Thaumatin-like protein | ||
| 126 | Act d 5 | N | f | Kiwellin | ||
| 127 | Act d 8 | R | f | PR-10 | ||
| 128 | Apple | Mal d 1 | R | f | PR-10 | |
| 129 | Peach | Pru p 1 | R | f | PR-10 | |
| 130 | Pru p 3 | R | f, skin | nsLTP, type 1 | ||
| 131 | Celery | Api g 1 | R | f | PR-10 | |
| 132 | Pineapple | Ana c 2.0101 | N | CCD | f, inh | Bromelain ( MUXF3, CCD-Marker) |
| 133 | Cow’s Milk | Bos d 4 | N | f | Alpha-lactalbumin | |
| 134 | Bos d 5 | N | f | Beta-lactoglobulin | ||
| 135 | Bos d 6 | N | f, inh | Serum Albumin | ||
| 136 | Bos d 8 | N | f | Caseins | ||
| 137 | Bos d Lactoferrin | N | f | Transferrin | ||
| 138 | Bos d 4 | N | f | Alpha-lactalbumin | ||
| 139 | Bos d 5 | N | f | Lipocalin (Beta-lactoglobulin) | ||
| 140 | Bos d 6 | N | f, inh | Serum Albumin | ||
| 141 | Bos d 8 | N | f | Caseins (α-, β-, κ-casein) | ||
| 142 | Bos d 9 | R | f | Caseins (αS1-casein) | ||
| 143 | Bos d 10 | R | f | Caseins (αS2-casein) | ||
| 144 | Bos d 11 | R | f | Caseins (β -casein) | ||
| 145 | Bos d 12 | R | f | Caseins (κ-casein) | ||
| 146 | Bos d Lactoferrin | N | f | Transferrin | ||
| 147 | Egg white | Gal d 1 | N | f | Ovomucoid | |
| 148 | Gal d 2 | N | f | Ovalbumin | ||
| 149 | Gal d 3 | N | f | Ovotransferrin (Conalbumin) | ||
| 150 | Egg yolk/chicken | Gal d 5 | N | f, inh | Serum Albumin | |
| 151 | Codfish | Gad c 1 | R | f | Parvalbumin | |
| 152 | Shrimp | Pen m 1 | N | f | Tropomyosin | |
| 153 | Pen m 2 | N | f | Arginine Kinase-like protein | ||
| 154 | Pen m 4 | N | f | Calcium-binding proteins (Sarcoplasmic) | ||
| 155 | Bee | Api m 1 | R | syst | Insect venom, Phospholipase A2 | |
| 156 | Api m 1 | R | syst | Insect venom, Phospholipase A2 | ||
| 157 | Api m 2 | R | syst | Insect venom, Hyaluronidase | ||
| 158 | Api m 4 | N | syst | Melittin | ||
| 159 | Api m 4 | N | syst | Melittin | ||
| 160 | Wasp | Ves v 1 | R | syst | Insect venom, Phospholipase A1 | |
| 161 | Ves v 5 | R | syst | Insect venom, Antigen 5 | ||
| 162 | Ves v 5 | R | syst | Insect venom, Antigen 5 | ||
| 163 | Paper wasp | Pol d 5 | R | syst | Insect venom, Antigen 5 | |
| 164 | Bacterial Toxins | Sem | R | skin, inh | ||
| 165 | SspB | R | skin, inh | |||
| 166 | Sec | R | skin, inh | |||
| 167 | HlgC | R | skin, inh | |||
| 168 | Tsst | R | skin, inh | |||
| 169 | Hlb | R | skin, inh | |||
| 170 | Plc | R | skin, inh | |||
| 171 | GlpQ | R | skin, inh | |||
| 172 | Hla | R | skin, inh | |||
| 173 | Ssl11 | R | skin, inh | |||
| 174 | Sei | R | skin, inh | |||
| 175 | Seb | R | skin, inh | |||
| 176 | Control-protein | MBP | R | - | Maltose binding protein | |
| Version 2 of the MeDALL-Chip (exchanged allergens) | ||||||
|---|---|---|---|---|---|---|
| 164 | Olive | Ole e 5 | R | inh | Superoxide dismutases | |
| 165 | Ole e 6 | R | inh | Oleaceae group 6 | ||
| 166 | Ole e 8 | R | inh | Calcium-binding proteins (Polcalcin) | ||
| 167 | Ole e 10 | R | inh | Glycosyl hydrolases | ||
| 168 | Wheat | Tri a gamma gliadin (GG1) | R | f | Gamma-gliadins | |
| 169 | Peptide 4 | R (peptide) | f | Peptides from gamma-gliadin 1 | ||
| 170 | Peptide 18 | R (peptide) | f | Peptides from gamma-gliadin 1 | ||
| 171 | Clone 79 | R | f | Gamma-gliadins | ||
| 172 | Clone 85 | R | f | Gamma-gliadins | ||
| 173 | Clone 110 | R | f | Gamma-gliadins (Fragment) | ||
| 174 | Tri a bA | R | f | beta-Amylases | ||
| 175 | Avenin-like protein | R | f | Avenin | ||
| 176 | Human | htTG2 | R | - | Human tissue transglutaminase | |