Table 2.
Results of the detection of dilution series of monoclonal IgE and IgG1. Dilution series of a monoclonal human IgE specific to Bet v 1 were tested by the MeDALL allergen-chip (left top) or Bet v 1-ImmunoCAP (right). A dilution series of a monoclonal human IgG1 specific to Phl p 2 was tested by the MeDALL allergen-chip (left bottom). Mean results and standard deviations of triplicate measurements are shown in ISU and ISU-G, respectively, for the microarray and in UA/ml for ImmunoCAP. For each dilution, variation coefficients (%CV) and signal-to-noise ratios (SNR) are shown. Orange boxes at the bottom of every table display results obtained for the sample diluent used for diluting the monoclonal antibodies.
| MeDALL-chip | ||||||||
|---|---|---|---|---|---|---|---|---|
| Bet v 1-specific monoclonal IgE | ||||||||
| IgE-conc. (ng/ml) |
ISU | %CV | mean %CV |
SD %CV |
SNR | mean SNR |
SD SNR |
|
| Mean | SD | |||||||
| 640 | 132.02 | 2.26 | 1.7 | 7.7 | 5.5 | 58.5 | 22.7 | 21.2 |
| 320 | 102.88 | 16.36 | 15.9 | 6.3 | ||||
| 32 | 23.30 | 1.83 | 7.9 | 12.7 | ||||
| 3.2 | 3.12 | 0.29 | 9.3 | 10.8 | ||||
| 1.6 | 1.67 | 0.07 | 4.0 | 25.1 | ||||
| 0.8 | 0.97 | 0.24 | 24.1 | 14.1 | 6.8 | 4.1 | 8.5 | 3.9 |
| 0.4 | 0.51 | 0.05 | 9.3 | 10.7 | ||||
| 0.2 | 0.28 | 0.04 | 12.4 | 8.1 | ||||
| 0.1 | 0.18 | 0.03 | 17.5 | 5.7 | ||||
| 0.05 | 0.14 | 0.01 | 7.1 | 14.0 | ||||
| 0 | 0.10 | 0.01 | 10.0 | 10.0 | ||||
| Phl p 2-specific monoclonal IgG1 | ||||||||
|---|---|---|---|---|---|---|---|---|
| IgG-conc. (ng/ml) |
ISU-G | %CV | mean %CV |
SD %CV |
SNR | mean SNR |
SD SNR |
|
| Mean | SD | |||||||
| 1000 | 181.22 | 15.12 | 8.3 | 14.9 | 7.9 | 12.0 | 8.1 | 3.6 |
| 500 | 161.01 | 16.26 | 10.1 | 9.9 | ||||
| 100 | 117.53 | 17.70 | 15.1 | 6.6 | ||||
| 10 | 31.61 | 8.21 | 26.0 | 3.8 | ||||
| 1 | 4.82 | 1.25 | 25.8 | 16.3 | 10.4 | 3.9 | 21.0 | 34.4 |
| 0.4 | 2.33 | 0.03 | 1.2 | 82.4 | ||||
| 0.3 | 1.64 | 0.31 | 18.6 | 5.4 | ||||
| 0.2 | 1.11 | 0.12 | 10.8 | 9.3 | ||||
| 0.1 | 0.59 | 0.15 | 25.0 | 4.0 | ||||
| 0 | 0.02 | 0.01 | 50.0 | 2.0 | ||||
| Bet v 1 ImmunoCAP | ||||||||
|---|---|---|---|---|---|---|---|---|
| Bet v 1-specific monoclonal IgE | ||||||||
| IgE-conc. (ng/ml) |
UA/ml | %CV | mean %CV |
SD %CV |
SNR | mean SNR |
SD SNR |
|
| Mean | SD | |||||||
| 312.5 | 91.20 | 3.35 | 3.7 | 2.7 | 1.4 | 27.3 | 50.1 | 33.6 |
| 39.1 | 10.57 | 0.29 | 2.7 | 36.6 | ||||
| 19.5 | 5.49 | 0.26 | 4.7 | 21.4 | ||||
| 7.8 | 2.16 | 0.05 | 2.1 | 47.1 | ||||
| 3.9 | 1.14 | 0.01 | 0.9 | 114.0 | ||||
| 2.0 | 0.63 | 0.01 | 1.8 | 54.3 | ||||
| 1.0 | 0.36 | 0.01 | 3.2 | 2.3 | 2.0 | 30.9 | 29.0 | 2.7 |
| 0.5 | 0.22 | 0.00 | 0.0 | - | ||||
| 0.2 | 0.16 | 0.01 | 3.7 | 27.1 | ||||
| 0 | 0.09 | 0.01 | 6.2 | 16.2 | ||||