Table 1. Summary of Illumina sequencing, assembly and mapping.
520 leaves | % | 520 roots | % | 592 leaves | % | 592 roots | % | Sum | % | |
---|---|---|---|---|---|---|---|---|---|---|
Total no of reads | 207,824,532 | 168,557,233 | 187,381,473 | 205,123,179 | 768,886,417 | |||||
Total no of bases (Gbp) | 20.8 | 16.9 | 18,7 | 20.5 | 76.9 | |||||
No of reads after trimming | 174,156,958 | 148,667,480 | 168,291,008 | 190,426,984 | 681,542,430 | |||||
Average read length after trimming | 98.4 | 98.5 | 97.9 | 98.7 | ||||||
Total mapped readsa | 64,492,170 | 37.0b | 35,511,470 | 23.9b | 38,630,740 | 23.0b | 39,889,518 | 20.9b | 178,523,898 | 26.2b |
Total unmapped readsc | 109,664,788 | 63.0b | 113,156,010 | 76.1b | 129,660,268 | 77.0b | 150,537,466 | 79.1b | 503,018,532 | 73.8b |
Multi-position matchese | 19,530,798 | 30.3d | 8,648,426 | 24.4d | 9,284,534 | 24.0d | 10,379,248 | 26.1d | 47,843,006 | 26.8d |
Unique matchesf | 44,961,372 | 69.7d | 26,863,044 | 75.6d | 29,346,206 | 76.0d | 29,510,270 | 74.0d | 130,680,892 | 73.2d |
aNumber of filtered sequencing reads that were aligned to the contigs in the filtered assembly.
bRelative to the number of reads after trimming.
cSequencing reads that could not be aligned to the contigs in the filtered assembly.
dRelative to the number of mapped reads.
eSequencing reads that aligned to two or more positions in the contigs in the filtered assembly.
fSequencing reads aligned to only one position in the contigs in the filtered assembly.