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. 2015 Dec 21;16:1087. doi: 10.1186/s12864-015-2315-5

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© Du et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 12 — Alternative splicing and tissue-specific expression of various GA metabolism pathway genes in S. miltiorrhiza. a–d: Gene structures of SmKO (a), SmGA20ox3 (b), SmGA2ox3 (c), SmGA2ox11 (d) and their splice variants. Filled boxes represent exons. Lines represent introns. e–h: Fold changes of SmKOv1 (e), SmGA20ox3v (f), SmGA2ox3v (g) and SmGA2ox11v1 (h) in roots (Rt), stems (St), leaves (Le) and flowers (Fl) of S. miltiorrhiza plants. The expression levels were analyzed using the quantitative RT-PCR method. Expression level in leaves was arbitrarily set to 1 and the levels in other tissues were given relative to this. Error bars represent standard deviations of mean value from three biological and four technical replicates. ANOVA (analysis of variance) was calculated using SPSS. P < 0.05 was considered statistically significant