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. 2015 Dec 21;10:69. doi: 10.1186/s13024-015-0065-0

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© O’Brien et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Beclin 1 localizes to endosomes in neurons. Representative images showing colocalization of beclin 1 with (a) Rab5-GFP, colocalization coefficient 0.49 ± 0.03, (b) Rab7-GFP, colocalization coefficient 0.50 ± 0.03 or (c) Golgin 97, colocalization coefficient 0.13 ± 0.01 in primary hippocampal neurons. Regions in the red boxes are enlarged below. Intensity traces (offset white lines) are plotted at right. Scale bars are 10 μm. Colocalization coefficients are the combined results of 2–3 independent experiments (n = 28-30 total fields/group).