Fig. 1.

Immunolocalization of CsCBs in C. sinensis worms. Sectioned worms were deparaffinized and incubated with anti-CsCBs sera (1: 400). Pre-immune rat serum was applied as a negative control. The sections were incubated with Cy3 conjugated goat anti-rat IgG secondary antibody (1: 400) in dark and imaged using an Axio Imager Z1 fluorescent microscope. 1–5 indicated anti-CsCB1 serum, anti-CsCB2 serum, anti-CsCB3 serum, anti-CsCB4 serum and pre-immune serum, respectively. a, b Metacercaria. c, d Cercaria. e, f Adult worm. a, c, e Images under fluorescent objective. b, d, f Images in bright field