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. 2015 Dec 21;8:647. doi: 10.1186/s13071-015-1248-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — The effect of yCsCB4 on human MHCC-97H and RBE cells. a Cell proliferation level measured by CCK-8 assay. b Cell cycle analysis by flow cytometry, the percentage of cells in the G2/S period was analyzed and quantified using FlowJo software. c, d Cell migration of MHCC-97H cells shown by wound-healing assay. Cells were observed using light microscope under 10X objective. e, f Cell migration of RBE cells shown by wound-healing assays; cells were observed using a light microscope under 10X objective. Generally, MHCC-97H cells or RBE cells were incubated with 1 μg/ml of yCsCB4 or PBS for 24 h and assays were performed. Assays were performed in triplicate. Relative cell migration level was calculated by normalizing to cell migration level at 0 h. *P < 0.05, ***P < 0.001, compared to PBS control