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. 2015 Dec 21;8:647. doi: 10.1186/s13071-015-1248-9

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© Chen et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Cell migration and invasion triggered by CsCB4 in transwell assay. MHCC-97H cells (a) or RBE cells (b) were suspended in serum-free media. Cells were incubated with 1 μg/ml of yCsCB4 or PBS for 24 h. Invasion assays were performed using matrigel-coated membranes. Migration assay was similar to the invasion assay, except that the upper side of the membranes was not coated with the matrigel. Cells attached to the lower surface of the membranes at 24 h were counted under a light microscope. Ten random visual fields were selected to quantify the migration and invasion. ***P < 0.001, compared to PBS control