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. 2015 Dec 21;10(12):e0145272. doi: 10.1371/journal.pone.0145272

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© 2015 Dobbins et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 7 — U251 cells were infected at an MOI of 1 VP/cell and then fixed at the times indicated with PBS containing 10% formalin, and mounted with Vectashield with DAPI. Fluorescent signal for pIX-RFP was detected by fluorescent microscopy (all magnifications at 1,000 × except 72h at 600 ×). Arrows indicate virus particle specs in the cytoplasm by 24 hpi, in the nucleus and vacuoles by 36 hpi; viral specs released from the nucleus into the cytoplasm by 96 hpi, and released from the cell by 120 hpi.