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. 2015 Dec 21;8:222. doi: 10.1186/s13068-015-0404-y

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© Madhaiyan et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Characterization of nitrogenase. a, b Acetylene reduction activity in vitro. Methylobacterium L2-4 was cultured in N-free medium containing various combination of co-factors (Fe/Mo/V) or concentrations of NH₄Cl. a Effects of co-factors. b Sensitivity to ammonium ions. c Nitrogenase activity of in planta. Seedlings of various crops were inoculated with L2-4 strain and AR assays were done 20 days after inoculation. Each value represents mean ± standard deviation (SD) of three replicates