Table 1.
Distribution of nitrogen-fixing and endoglucanase-positive strains originating from different media
| Media | Medium | Total number of isolatesa | Positive isolates (%) | ||
|---|---|---|---|---|---|
| nifH b | ARAc | Endoglucanased | |||
| Rich media | M869 | 257 | 132 (51.4) | 81 (29.5) | 98 (38.1) |
| KB | 354 | 206 (58.2) | 143 (40.4) | 155 (43.8) | |
| Heterotrophic media | R2A | 189 | 112 (59.3) | 58 (30.7) | 75 (39.7) |
| Minimal media | AMS | 106 | 80 (75.5) | 49 (46.2) | 33 (31.1) |
| N-free media | Nfb | 101 | 68 (67.3) | 45 (45.6) | 78 (77.2) |
| Baz | 10 | 5 (50.0) | 5 (50.0) | 9 (90.0) | |
aTotal number of isolates selected for 16S rRNA gene sequencing and phylogeny
bPCR amplification of nifH gene fragments was performed using specific degenerate primers
cAcetylene reduction activity in pure culture was measured by GC
dPlate assays for endoglucanase activity using KM solid medium with 0.2 % CMC were spot inoculated with endophytes and incubated at 30 °C for 3 days