Fig. 6.

Enhanced anti-tumor effect of rNDV-18HL. a Orthotopic transplantation HCC nude mice were treated with rNDV-18HL, NDV Italien, or normal saline through tail intravenous injection twice weekly for 3 weeks. At 24 h post-injection, three mice of each group were sacrificed to investigate the metastatic foci in the liver. The representative images were shown by gross observation (upper) and Ki-67 staining (bottom). b The number of metastatic foci was counted (three Ki-67 staining slides were selected randomly for each sample, and only separated metastatic foci bigger than 50 μm was counted) and indicated as means ± SD. * P < 0.05, ** P < 0.01. c Orthotopic transplantation HCC nude mice were treated with rNDV-18HL, cHAb18, NDV Italien, or normal saline through tail intravenous injection twice weekly for 3 weeks. The number of mice in each group was 11. The treated mice were monitored daily and the Kaplan-Meier survival curve was plotted and analyzed by log-rank test. d The body weights were recorded every 3 days after treatment