Table 1.
Primer names | Sequences (5' - 3') |
---|---|
18 L-F | TTAGAAAAAAATACGGGTAGAAATAAGCCACCATGGACTCACATACTC |
18 L-R | TATTCCGGATTAACACTCTCCCCTGTTGAA |
18H-F | ATATCCGGAGCCACCATGAACTTCGGGCTGAGC |
18H-R | TTCTACCCGTATTTTTTTCTAAGTGATAGTGATCATTTACCCGGAGAC |
VT-F | GACGGGATAACTCTGAGG |
VT-R | GTATGCCAACAAGGTCGC |
aUnderline indicated the overlap regions in overlap PCR