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. 2015 Dec 21;15:295. doi: 10.1186/s12870-015-0670-7

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© Basu et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Subcellular localization of transiently expressed GALT3-YFP, GALT4-YFP, and GALT6-YFP in N. tabacum. GALT3-YFP, GALT4-YFP, and GALT6-YFP fusion constructions were expressed under the control of the CaMV 35S promoter in N. tabacum. Transiently expressed GALT3-YFP, GALT4-YFP, and GALT6-YFP co-localized with sialyl transferase (ST)-GFP fusion protein (a Golgi marker), but not with HDEL-GFP fusion protein (an ER marker). These constructs were examined by laser-scanning confocal microscopy under fluorescent and white light, and the fluorescent images were merged to observe co-localization. Size bar = 10 μm