Table 5.
Quantification of total sugars from WT and galt mucilage sequentially extracted using ammonium oxalate, 0.2 N NaOH, and 2 N NaOH
| Extracta | |||
|---|---|---|---|
| Genotype | Ammonium oxalate | 0.2 N NaOH | 2 N NaOH |
| WT | 0.85 ± 0.05 | 1.04 ± 0.03 | 0.81 ± 0.04 |
| galt1-2 | 0.83 ± 0.07 | 1.05 ± 0.05 | 0.84 ± 0.03 |
| galt2-1 | 0.95 ± 0.03b | 1.12 ± 0.04b | 0.61 ± 0.05b |
| galt2-2 | 0.97 ± 0.70b | 1.19 ± 0.03b | 0.54 ± 0.06b |
| galt3-1 | 1.30 ± 0.09c | 1.29 ± 0.05c | 0.53 ± 0.05b |
| galt3-2 | 1.28 ± 0.05c | 1.27 ± 0.07c | 0.52 ± 0.07b |
| galt4-1 | 0.88 ± 0.20 | 1.09 ± 0.02 | 0.79 ± 0.01 |
| galt4-2 | 0.90 ± 0.60b | 1.01 ± 0.06 | 0.73 ± 0.03 |
| galt5-1 | 0.95 ± 0.10b | 1.17 ± 0.05b | 0.63 ± 0.05b |
| galt5-2 | 0.90 ± 0.08b | 1.20 ± 0.07c | 0.60 ± 0.04b |
| galt6-1 | 1.25 ± 0.09c | 1.30 ± 0.07c | 0.55 ± 0.05b |
| galt6-2 | 1.31 ± 0.05c | 1.29 ± 0.04c | 0.61 ± 0.08b |
| galt2galt5 | 1.40 ± 0.09d | 1.36 ± 0.08d | 0.47 ± 0.05d |
aIntact seeds were extracted sequentially with 0.2 % ammonium oxalate, 0.2 N NaOH and 2 N NaOH, neutralized, and assayed by the phenol-sulfuric acid method against glucose standards. The results are shown as μg/mg of seeds. Analyses were performed in triplicate and results are given as μg/mg seed ± SE. All genotypes were grown, harvested, and stored together. Letters ‘b’ ‘c’ and ‘d’ denote a significantly difference from the wild type (Dunnett’s test, P <0.05; P <0.01; P <0.001 respectively)