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. 2015 Dec 21;14:202. doi: 10.1186/s12934-015-0394-2

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© Yang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Construction of recombinant plasmid pSIP409-ACEIP. a Designed peptide sequences according to the synthesized oligonucleotides. Black bold, peptides of tuna frame protein; Black bold italic, arginine linker; Underlined, peptides of limanda aspera frame protein; Black bold underlined, His tag. b Illustration of pSIP409-ACEIP. Encoding sequences of angiotensin-converting enzyme inhibitory peptide (ACEIP) were digested by NcoI and HindIII and were ligated with pSIP409 that was digested with the same enzymes. 256rep replication origin for Lactobacillus, ermL erythromycin-resistance marker, P _sppIP and P _orfX inducible promoters, sppK and sppR histidine protein kinase and response regulator, respectively