Fig. 2.

Phylogenetic analysis of SMase D sequences by ML method. Phylogenetic tree based on the ML method (lnL = −46,243.0294). A GTR model was chosen (BIC score = 72,153; lnL = −33,053.48462). Gamma distribution (G parameter = 2.5232). The rate variation model allowed certain sites to be evolutionarily invariable (I). Loxosceles SMase D Class I isoforms are indicated in clades L and G (red)