FIGURE 1.

Long-term hyphal-baiting (“transfer-enrichment”) approach. (A) Top-view and (B) side-view. The fungus was inoculated in the lid of an Eppendorf Cup containing Malt extract agar. It overgrew the plastic rim which separated the nutrient containing compartment (Malt extract agar) in the lid from the nutrient-poor Phytagel in the Petri dish. Subsequently, the microcosm was colonized by the fungal hyphae. Bacteria were distributed over the Phytagel compartment after colonization by the hyphae and strains that became enriched appeared as a biofilm or small colonies along fungal hyphae (red). (C) Regular transfers were done to promote the enrichment of those bacteria that can exploit fungal hyphae for carbon.