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. 2015 Nov 6;16(12):1656–1663. doi: 10.15252/embr.201540861

Figure 4. Lysosomal function inhibition affecting TLR8 activity is anti‐inflammatory upon both, Gram‐positive and Gram‐negative bacterial infection ex vivo .

Figure 4

  1. Cytokine release of 3‐day differentiated THP‐1 cells of the indicated genotypes upon TLR2 blockade (T2.5) and challenge with TLR ligands, heat‐inactivated (hi) or viable bacteria.
  2. PBMC activity upon endosome function inhibition (chloroquine) and TLR2 blockade (T2.5) followed by TLR ligand challenge or bacterial infection.
  3. Activity of whole blood culture upon endosome function inhibition (chloroquine) and challenge with TLR ligands, heat‐inactivated (hi), or viable bacteria.
Data information: Graphs show mean ± SD; n = 3; *P ≤ 0.05; **P ≤ 0.01; unpaired t‐test; –, unchallenged; P3C, Pam3 CSK 4; n.d., not detected; triangles, decreasing doses of exponentially growing bacteria gentamicin‐treated 1 h post‐infection.