Table I.
Primer sets used for RT-PCR.
| Gene | Primer sequence (5′→3′) | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
| GAPDH | Forward: GTGGTCTCCTCTGACTTCAACA | ||
| Reverse: CTCTTCCTCTTGTGCTCTTGCT | 62 | 210 | |
| OCT4 | Forward: GACAACAATGAGAACCTTCAGGAGA | ||
| Reverse: TTCTGGCGCCGGTTACAGAACCA | 62 | 218 | |
| SOX2 | Forward: AACCAAGACGCTCATGAAGAAG | ||
| Reverse: GCGAGTAGGACATGCTGTAGGT | 62 | 341 | |
| c-Myc | Forward: TCGGATTCTCTGCTCTCCTC | ||
| Reverse: CGCCTCTTGACATTCTCCTC | 62 | 413 | |
| KLF4 | Forward: ATTCTCTCCAATTCGCTGACCC | ||
| Reverse: TTCAGCACGAACTTGCCCAT | 62 | 376 | |
| NANOG | Forward: ATAGCAATGGTGTGACGCAG | ||
| Reverse: GATTGTTCCAGGATTGGGTG | 62 | 219 | |
| REX1 | Forward: CTGAAGAAACGGGCAAAGAC | ||
| Reverse: GAACATTCAAGGGAGCTTGC | 58 | 344 | |
| LIN28 | Forward: GCTCCGTGTCCAACCAGCAG | ||
| Reverse: TTTCCTTTTGGCCGCCTCTC | 58 | 376 | |
| GD2 synthase | Forward: CCAACTCAACAGGCAACTAC | ||
| Reverse: GATCATAACGGAGGAAGGTC | 59 | 230 | |
| DLX5 | Forward: ACCATCCGTCTCAGGAATCG | ||
| Reverse: ACCTTCTCTGTAATGCGGCC | 60 | 384 | |
| CBFA1 | Forward: TTGCAGCCATAAGAGGGTAG | ||
| Reverse: GTCACTTTCTTGGAGCAGGA | 58 | 470 | |
| PPARG | Forward: TCTCTCCGTAATGGAAGACC | ||
| Reverse: GCATTATGAGACATCCCCAC | 55 | 474 | |
| C/EBPA | Forward: CCAAGAAGTCGGTGGACAAGAA | ||
| Reverse: TCATTGTCACTGGTCAGCTCCA | 62 | 145 | |
| BMP7 | Forward: CCAACGTCATCCTGAAGAAATAC | ||
| Reverse: GCTTGTAGGATCTTGTTCATTGG | 60 | 271 | |
| SOX9 | Forward: GGTTGTTGGAGCTTTCCTCA | ||
| Reverse: TAGCCTCCCTCACTCCAAGA | 61 | 400 | |
| HLA-ABC | Forward: CAGATACCTGGAGAACGG | ||
| Reverse: TGGCCTCATGGTCAGAGA | 56 | 96 | |
| HLA-DR | Forward: CCCCACAGCACGTTTCTTG | ||
| Reverse: CCGCTGCACTGTGAAGCTCT | 60 | 274 | |
| HLA-G | Forward: GCGGCTACTACAACCAGAGC | ||
| Reverse: GCACATGGCACGTGTATCTC | 58 | 900 | |
| IL10 | Forward: ACCTGGTAGAAGTGATGCCCCAGGCA | ||
| Reverse: CTATGCAGTTGATGAAGATGTCAA | 58 | 237 | |
| TNFAIP6 | Forward: GGTGTGTACCACAGAGAAGCA | ||
| Reverse: GGGTTGTAGCAATAGGCATCC | 60 | 284 | |
| TSG-6 | Forward: GGTGTGTACCACAGAGAAGCA | ||
| Reverse: GGGTTGTAGCAATAGGCATCC | 60 | 284 | |
| IL6 | Forward: ATGAACTCCTTCTCCACAAGC | ||
| Reverse: GTTTCTGCCAGTGCCTCTTTG | 60 | 264 | |
| TGFB1 | Forward: GAGGTGACCTGGCCACCATT | ||
| Reverse: TCCGCAAGGACCTCGGCTGG | 55 | 194 | |
| INHBA | Forward: GATGTACCCAACTCTCAGCCA | ||
| Reverse: GCCGATGTCCTTGAAACTGAC | 55 | 866 |
SOX2, sex determining region Y-box 2; DLX5, distal-less homeobox 5; C/EBPA, CCAAT/enhancer-binding protein alpha; BMP7, bone morphogenetic protein 7; IL, interleukin ; TGFB1, transforming growth factor beta 1. OCT4, octamer-binding transcription factor 4; KLF4, Krüppel-like factor 4; DLX5, distal-less homeobox 5; CBFA1, core-binding factor alpha (1); PPARG, proliferator-activated receptor gamma; TNFAIP6, tumor necrosis factor, alpha-induced protein 6; TSG-6, tumor necrosis factor-stimulated gene 6 (TSG-6); IHHBA, Activin A, inhibin beta A.