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. 2015 Sep 21;37(1):21–28. doi: 10.3892/ijmm.2015.2351

Figure 2.

Figure 2

(a) Culture of HaCaT keratinocytes (magnification, ×600), which express both estrogen receptor (ER)-α and -β mainly in the cell nuclei; cytoskeleton is stained with high-molecular-weight keratins (HMWK), cell nuclei are visualized by DAPI. (b) HaCaT keratinocytes cultured under the influence of selective ER agonists; first line of horizontal panels (A1-A3, magnification, ×200): detection of the proliferation marker Ki-67 and wide-spectrum keratin; second horizontal panel (B1-B3, magnification, ×200): presence of keratin-19 (marker of poorly differentiated keratinocytes with stem-like phenotype) and Sox-2 (stem cels marker); third horizontal panel (C1-C3, magnification, ×100): positivity for keratin-14 (marker of poorly differentiated keratinocytes) and wide-spectrum keratin; fourth horizontal panel (D1-D3, magnification, ×100): expression of keratin-10 (marker of differentiated keratinocytes) and wide-spectrum keratin; fifth horizontal panel (E1-E3, magnification, ×100): presence of keratin-19 and galectin-1 (Gal-1). PPT-, 4,4′,4″-(4-propyl [1H] pyrazole-1,3,5-triyl)-trisphenol, a selective ER-α agonist; DPN, 2,3-bis(4-hydroxyphenyl)-propionitrile, a selective ER-β agonist; *P<0.05 and **P<0.01. C, control; K, wide-spectrum keratin.