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. 2015 Nov 13;37(1):99–107. doi: 10.3892/ijmm.2015.2410

Figure 2.

Figure 2

Expression of high-mobility group box 1 (HMGB1) and Toll-like receptor 4 (TLR4) and activation of nuclear factor-κB (NF-κB) in pancreatic tissue from mice after L-arginine administration or sham injection. (A) Serum HMGB1 concentrations [(a) ELISA] were assessed in the sham and severe acute pancreatitis (SAP) groups. The expression of HMGB1 was assessed using the mRNA [(b) qPCR] and protein [(c) western blot analysis] levels in the pancreas tissue from the sham and SAP groups. (B) The expression of TLR4 was assessed with the mRNA [(a) qPCR] and protein [(b) western blot analysis] levels in the pancreatic tissue from the sham and SAP groups. (C) The activation of NF-κB was assessed from the nuclear p65 [(a) western blot analysis] and its downstream, relative levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) [(b) ELISA] in the pancreatic tissue from the sham and SAP groups. *P<0.01 compared to the sham group (control group). The relative levels of HMGB1 and TLR4 protein and mRNA compared to the control group are normalized to β-actin. The relative levels of nuclear p65 protein compared to the control group are normalized to histone H3. The relative levels of TNF-α and IL-1β compared to the control group are normalized to the total protein concentration of each sample. Data are expressed as the means ± SD (n=6/group). Blots shown are from a representative experiment that was repeated 3 times with similar results (n=6/group). Sham, sham injection.