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. 2015 Nov 13;37(1):99–107. doi: 10.3892/ijmm.2015.2410

Figure 4.

Figure 4

Toll-like receptor 4 (TLR4) expression and nuclear factor-κB (NF-κB) activation in pancreatic tissue after recombinant human high-mobility group box 1 (rhHMGB1) administration. (A) The expression of TLR4 was assessed using the mRNA [(a) qPCR] and protein [(b) western blot analysis] levels in the pancreatic tissue from the sham, low-dose of HMGB1 (HM-LD) and high-dose of HMGB1 (HM-HD) groups at 48 h after rhHMGB1 administration. (B) The activation of NF-κB was assessed from the nuclear p65 [(a) western blot analysis] and its downstream, relative levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) [(b) ELISA] in the pancreatic tissue of the sham, HM-LD and HM-HD groups. *P<0.01 compared to the sham group (control group); #P<0.05 compared to the HM-LD group. The relative levels of the TLR4 protein and mRNA compared to the control group are normalized to β-actin. The relative levels of nuclear p65 protein compared to the control group are normalized to histone H3. The relative levels of TNF-α and IL-1β concentrations compared to the control group are normalized to the total protein concentration of each sample. Data are expressed as the means ± SD (n=6/group). Blots shown are from a representative experiment that was repeated 3 times with similar results (n=6/group). Sham, sham injection.