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Effects of tuna heart (TH)-H2O fraction on phosphatidylinositol 3-kinase (PI3K)/Akt and matrix metalloproteinase (MMP) gene expression. Hs27 cells were cultured in 100-mm dishes with TH-H2O fraction (25, 50 and 100 µg/ml) for 24 h, after which time cellular protein and total RNA were isolated. (A) Protein levels were assessed by western blot analysis. (B) RT-PCR analysis was achieved by electrophoresis in a 1% agarose gel stained with Red Safe nucleic acid staining solution.
