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. Author manuscript; available in PMC: 2016 Nov 21.
Published in final edited form as: Analyst. 2015 Nov 21;140(22):7748–7760. doi: 10.1039/c5an00977d

Figure 8.

Figure 8

SVM-DA cross-validation (CV) predicted plots for (A) high, and (B) low NA-EIV assays. A total of 80 spectra are represented in this plot, with 20 replicates in each group. Each colored symbol represents the SVM CV predicted class membership for an individual SERS spectrum after incubation of synthetic RNA strains at 37°C for 2 hours with DNA probe. The colored symbol and sample numbers are identical to the samples described in Figures 6 and 7.

(A) The colored symbols represents SERS spectra containing: the high NA-EIV RNA strains of A/ck/Hubei/327/2004/H5N1 ( Inline graphic), and A/WSN/33/H1N1 ( Inline graphic) and the low NA-EIV RNA strains of A/Gs/Gd/1/96/H5N1 ( Inline graphic) incubated with high NA-EIV DNA probe. High DNA probe with MCH spacer was used as a control ( Inline graphic).

(B) The colored symbols represents SERS spectra containing: the low NA-EIV RNA strains of A/Gs/Gd/1/96/H5N1 ( Inline graphic) and the high NA-EIV RNA strains of A/ck/Hubei/327/2004/H5N1 ( Inline graphic), and A/WSN/33/H1N1 ( Inline graphic) incubated with low NA-EIV DNA probe. Low NA-EIV DNA probe with MCH spacer was used as control ( Inline graphic).