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. Author manuscript; available in PMC: 2016 Nov 21.

Published in final edited form as: Analyst. 2015 Nov 21;140(22):7748–7760. doi: 10.1039/c5an00977d

PLS-DA prediction plots for the DNA/RNA hybrid complexes prepared using RNAs isolated from laboratory influenza strains containing the NA stalk mutations. A total of 80 spectra are represented in each plot, with 20 replicates in each group. Each colored symbol represents the PLS predicted value for an individual SERS spectrum after incubation of RNA strains at 37°C for 2 hours with DNA probe.

(A–D) The colored symbols represents SERS spectra containing: A/WSN/33/H1N1 ( ), A/Vietnam/1203/2004/H5N1 ( ) and A/Anhui/1/2005/H5N1 ( ). DNA probe with MCH spacer was used as a control ( ).

Inline graphic — PLS-DA prediction plots for the DNA/RNA hybrid complexes prepared using RNAs isolated from laboratory influenza strains containing the NA stalk mutations. A total of 80 spectra are represented in each plot, with 20 replicates in each group. Each colored symbol represents the PLS predicted value for an individual SERS spectrum after incubation of RNA strains at 37°C for 2 hours with DNA probe.

(A–D) The colored symbols represents SERS spectra containing: A/WSN/33/H1N1 ( ), A/Vietnam/1203/2004/H5N1 ( ) and A/Anhui/1/2005/H5N1 ( ). DNA probe with MCH spacer was used as a control ( ).