. Author manuscript; available in PMC: 2015 Dec 22.

Published in final edited form as: Curr Protoc Hum Genet. 2011 Oct;0 19:Unit19.8. doi: 10.1002/0471142905.hg1908s71

Table 2.

Thermocycler program

State	Temperature	Time
Denaturation	95 °C	2 min
Amplification (35 cycles)	95 °C	20 sec
	61 °C	30 sec
	68 °C	10 min
End of Amplification/Incubation	68 °C	20 min
End of Amplification/Incubation	4 °C	∞