Figure 3.

Detection of inducible co‐stimulatory (ICOS)⁺ and ICOS⁻CD45RA⁺CD31⁺ recent thymic emigrant (RTE) regulatory T cells (T_regs), ICOS⁺ and ICOS⁻CD45RA⁺CD31⁻ mature naive (MN) T_regs, ICOS⁺ and ICOS⁻CD45RA⁻CD31⁺ memory T_regs and ICOS⁺ and ICOS⁻CD45RA⁻CD31⁻ memory T_regs within the total CD4⁺CD127^low+/−forkhead box protein 3 (FoxP3)⁺ T_reg pool during the normal pregnancy course and in the presence of pre‐eclampsia and haemolysis elevated liver enzymes low platelet (HELLP) syndrome. The percentage of total CD4⁺CD127^low+/−FoxP3⁺ T_regs within CD4⁺ T cells (a), the percentages of ICOS⁺ (b) and ICOS⁻ T_regs (c), ICOS⁺ and ICOS⁻CD45RA⁺CD31⁺ RTE T_regs (d,h), ICOS⁺ and ICOS⁻CD45RA⁺CD31⁻ MN T_regs (e,i), ICOS⁺ and ICOS⁻CD45RA⁻CD31⁺ memory T_regs (f,j) and ICOS⁺ and ICOS⁻CD45RA⁻CD31⁻ memory T_regs (g,k) within the total CD4⁺CD127^low+/−FoxP3⁺ T_reg pool were determined in healthy non‐pregnant fertile women (group 1, ), healthy pregnant women during their pregnancy course (groups 2–5, ◆), spontaneously term labouring women (group 6, ), 1 day postpartum (group 7, ◆) and in women with pre‐eclampsia (group 8, ) or HELLP syndrome (group 9, ). Significant differences concerning the percentages of the different T_reg subsets were detected between women during the normal pregnancy course (groups 2–5) and non‐pregnant women (group 1) or women with spontaneous term labour (group 6). In addition, significant differences were detected between healthy third‐trimester women (groups 4 and 5) and women with pre‐eclampsia (group 8) or HELLP syndrome (group 9).