Table 1.
Binding affinity of tristetraprolin (TTP) and human antigen R (HuR) to synthetic RNA fragments
| RNA | ARE sequence | nM binding affinity to TTP | nM binding affinity to HuR | Reference |
|---|---|---|---|---|
| AUUUAUUUAUUUA | 3·6 | 7, 8 | ||
| AUUUAUUUAUUUAUUUAUUUAUUUA | 0·5 | 0·50 | 9 | |
| UUAUUUAUU | 3·0 | 7, 8 | ||
| UAUUUAU | 19·0 | 7, 8 | ||
| UUUUUUUU | Not bound | 10 | ||
| UUUUUUUUU | 0·97 | 10 | ||
| UUUUUUUUUUUUU | 280·0 | 7, 8 | ||
| UUUUAUUUAUUUU | 3·2 | 7, 8 | ||
| UUUUAUUUUUUUU | 56·0 | 7, 8 | ||
| UUUUAUUUCUUUU | 83·0 | 7, 8 | ||
| UUUUAUUUGUUUU | 28·0 | 7, 8 | ||
| UUUUUUUUAUUUU | 54·0 | 7, 8 | ||
| UUUUCUUUAUUUU | 93·0 | 7, 8 | ||
| UUUUGUUUAUUUU | 28·0 | 7, 8 | ||
| UUAUUUUUU | 130·0 | 7, 8 | ||
| UUUUUUAUU | 120·0 | 7, 8 | ||
| UUUUAUAUUUU | 160·0 | 7, 8 | ||
| UUUUAUUAUUUU | 18·0 | 7, 8 | ||
| UUUUAUUUAUUUU | 3·2 | 7, 8 | ||
| UUUUAUUUUAUUUU | 6·4 | 7, 8 | ||
| UUUUAUUUUUAUUUU | 17·0 | 7, 8 | ||
| NNUUNNUUU | 0·96 | 10 | ||
| UAUUAUUUU | 1·14 | 10 | ||
| A AUUUAUUU | 1·01 | 10 | ||
| CUUUCUUUCUUUCUUUC | 0·96 | 10 | ||
| cFos | UAUUUAUAUUUUUAUUUUAUUU | 34 | 11 | |
| c‐Myc | UUA C C AUCUUUUUUUUUCUUUA | >1000 | 11 | |
| Cox‐2 | UAUUA AUUUA AUUAUUUA AUA AUAUUUAUAUUA A A | 13·6 | 10 | |
| GM‐CSF | AUUUAUUUAUUUAUUUAUUUA | 21 | 11 | |
| IL‐1β | UAUUUAUUUAUUUAUUUGUUUGUUUGUUUUAUU | 0·12 | 10, 12 | |
| IL‐2 | UAUUUAUUUA A AUAUUUA A AUUUUAUAUUUAUU | 44 | 9·5 | 10, 11, 12 |
| IL‐3 | UAUUUAUUUAUGUAUUUAUGUAUUUAUUUAUUUAU | 4 | 11 | |
| IL‐8 | UAUUUAUUAUUUAUGUAUUUAUUUA A | 1·09 | 10, 12 | |
| TNF‐α | AUUAUUUAUUAUUUAUUUAUUAUUUAUUUAUUUA | 2 | 0·35 | 10, 11, 12 |
| cFos | 3′UTR | 10 | 13 | |
| IL‐2 | 3′UTR | 32·77 | 10 | |
| TNF‐α | 3′UTR | 3·87 | 10 |
Dissociation constants (Kd) for TTP and HuR ARE interactions at 25°C. Data taken from published fluorescence anisotropy data. Adenosine residues are shown in red, cytosine and guanine residues are shown in blue and N (any) residues are shown in purple. ARE = adenosine–uridine‐rich element; GM‐CSF = granulocyte–macrophage colony‐stimulating factor; TNF‐α = tumour necrosis factor‐alpha; IL = interleukin.