Fig. 6.

mgtM translation promotes transcription termination by sequestering RARE. Epistasis analysis of mutations affecting Rho-dependent termination in the mgtCBR leader. Fluorescence levels exhibited by wild-type Salmonella (14028s) harboring a plasmid that contains a transcriptional fusion of the wild-type mgtCBR promoter and leader, or mgtCBR leader variants, to a promotorless gfp gene. The experiment was performed as described in the legend to Fig. 1B. Single mutations are shown with solid bars and double mutations are shown with dashed bars. (−) corresponds to wild-type Salmonella (14028s) harboring the plasmid vector pFPV25. Data correspond to the average from three independent experiments.