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. 2015 Nov 25;112(50):15408–15413. doi: 10.1073/pnas.1512832112

Fig. 2.

Fig. 2.

Intratumoral STING activation leads to systemic CD8 T-cell–mediated antitumor immunity that controls the growth of distant tumors. (A) Mice bearing a 5-d established s.c. B16F10 tumor were treated by i.t. injection of cGAMP (cGAMP-inj) or Lipofectamine alone (Ctrl-inj) followed by i.v. injection of B16F10 melanoma cells. After 10 d, mice were killed and lung metastases counted macroscopically. Photographs of representative lungs are depicted (Left). Each symbol represents an independent mouse (Right); data are combined from two experiments. ****P < 0.0001 by unpaired t test. (B and C) Mice bearing two s.c. B16F10 tumors in opposite flanks were treated by i.t. injection of cGAMP (cGAMP-inj) or Lipofectamine alone (Ctrl-inj) in only one tumor. CD8-depleting antibody or saline were injected i.p. Graph depicts tumor growth over time of contralateral not injected tumors (B) and injected tumors (C). Data represent the mean tumor volume ± SEM of 9–10 mice per group pooled from two experiments. *P < 0.05, **P < 0.01 by two-way ANOVA.