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. 2015 Nov 25;112(50):15408–15413. doi: 10.1073/pnas.1512832112

Fig. S2.

Fig. S2.

Intratumoral cGAMP injection promotes the generation of Ag-specific cytotoxic CD8 T cells that infiltrate the tumors. B16-WT or B16-OVA (if indicated) tumor cells were implanted s.c. into WT mice. (A and B) cGAMP (cGAMP-inj) or Lipofectamine alone (Ctrl-inj) was injected into tumors at day 5 and day 10. (A) Flow cytometry analysis of CD44 and CD62L expression gated on CD8 T cells infiltrating tumors at day 15. CD8 T cells from spleen of naive mice were used as control. (B) Tumor cell suspensions were cultured with Brefeldin A for 5 h in addition of PMA/Ionomycin if indicated. Data represent flow cytometry detection and quantification of IFNγ or Perforin expressing-CD8 T cells in tumor-derived cell suspension. *P < 0.05 by unpaired t test. (C and D) At day 4, 5 × 106 OT-I splenocytes were transferred intravenously. cGAMP (cGAMP-inj) or Lipofectamine alone (Ctrl-inj) was injected into the tumor at day 5 and day 10. Flow cytometry analysis and quantification of OVA-specific CD8 T cells in tumors at day 15 detected with Vα2 (C) or SIINFEKL tetramer staining (D). **P < 0.01 by unpaired t test.