Fig. S7.

Tumor extracts from cGAMP-injected tumor inhibit the growth of B16F10 cells in a type I IFN-dependent manner. Protein extracts were prepared from tumors lysed after they have been injected in vivo for 4 h with cGAMP or Lipofectamine (Ctrl). B16F10 cells were incubated with 300 µg/mL of tumor lysate proteins. Type I IFN signaling was blocked by adding 10 µg/mL of anti-IFNAR1 antibody (+) in the culture medium. As positive control, 10 units/mL of murine recombinant IFN-β was added to the culture (+). Data represent the percentage of cellular expansion compare with Ctrl conditions after 3 d of culture. Data are representative of at least three independent experiments. ****P < 0.0001 by unpaired t test.