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Effect of voltage on blockade of KcsA-Shaker-A54D channels by Hui1 and Hui1 mutants. Ki for each toxin was determined from −20 mV to 40 mV based on the fraction of unblocked current at equilibrium (Fun) and normalized to the value at 0 mV as in Fig. 3F. The change in Ki with voltage was due to changes in toxin off-rates (τoff). Some error bars are smaller than symbols.
