Skip to main content
. 2015 Nov 30;112(50):15414–15419. doi: 10.1073/pnas.1515978112

Fig. 3.

Fig. 3.

IL12RB1 mRNAs expressed in inflamed human lungs contain RDDs. Lung tissue from four different individuals (donors N1, N2, S1, and S2) was scored for the presence or absence of pulmonary inflammation by H&E analysis; tissue from areas adjacent to the sectioned areas were then used for IL12RB1 RDD identification by Ion Torrent and Sanger sequencing. (A and B) Representative 10× magnification images of (A) noninflamed lung tissue from donors N1 and S1 and (B) inflamed lung tissue from donors N2 and S2 (Br, bronchiole; Vs, blood vessel). Inset within the images B are 40× magnifications of the same sections, which demonstrate that inflammatory infiltrates make up both polymorphonuclear and mononuclear lineages. (C) Listed for each lung along the horizontal axis are the RDDs identified in IL12RB1 transcripts expressed in each lung. Each bar represents the total number of reads at that specific site in the IL12RB1 transcript; the RDD level at each specific site is indicated in the pink portion of each bar. IL12RB1 transcripts in donors N1 and S1 did not contain any RDDs differing from the reference sequence. Inset within C is a gel image of the IL12RB1 mRNA amplicons from each tissue sample alongside a positive control, as visualized by agarose gel electrophoresis. (D–K) Sanger sequencing traces of IL12RB1 mRNA clones from donor N2 containing the RDDs (D) A119G, (E) C168U, (F) A815G, (G) U1040C, (H) U1255C, (I) U1559C, (J) G1575A, and (K) C1767U.