Skip to main content
. 2015 Nov 4;112(50):E6852–E6861. doi: 10.1073/pnas.1518028112

Fig. 6.

Fig. 6.

Both the frequency of initiation and rate of DNA unwinding by RecQ increase with decreased dsDNA stability. (A) Helicase activity of RecQ (100 nM) as a function of temperature (20 °C to 47 °C) as measured by the dye-displacement assay (see Fig. S6 and SI Materials and Methods). (B) Steady-state ensemble rate of unwinding as function of temperature at: 1 mM (black circles), 2 mM (blue circles), and 5 mM (red circles) Mg(OAc)2. Single-molecule experiments: (C) rate of initiation and (D) rate of unwinding as function of temperature for RecQ (40 nM), with AF488SSB (60 nM); n = 5–14 forks at each temperature. Error bars represent SD.