Fig. 2.

CDR germ-lining illustration via surface space-fill plots of non-germ-line amino acid retention. (A) Heatmap (based on cocrystal structure PDB 4GLR) of non-germ-line residue content in the anti-pTau binding site for Par-pTau, Graft-pTau and C21-ABS-pTau. Residues matching human DP47/DPL16 germ lines are shown (spacefill) in gray, non-germ-line residues in orange (CDR-H3) or red (all other CDRs). The target pTau phospho peptide is included in green, illustrating the predominant retention of surface-exposed residues involved in the functional paratope in clone C21-ABS-pTau. Although cocrystal structures for anti-RAGE (B) and anti-A33 (C) were not available, structural modeling showed that the ABS process clearly defines residues that may be essential for antigen-binding function.