. 2015 Nov 30;112(50):15354–15359. doi: 10.1073/pnas.1510944112

Table S1.

CDR sequence, affinity, and stability characteristics of parental, grafted, and ABS-derived lead clones

	CDRL1^*	CDRL2	CDRL3	CDRH1	CDRH2	CDRH3	% germ line in CDRs	No. of FW mutations	IC50 (nM) HTRF	kDa (nM) SPR	% Agg 60 °C	Tm (°C)	% Loss in pH shock
DPK9/DP54	`RASQSISSYLN`	`AASSLQS`	`QQSYSTPLT`	`GFTFSSYWMS`	`ANIKQDGSEKYYVDSVKG`	n/a	100	n/a	n/a	n/a	n/a	n/a	n/a
CL-Hum-RAGE	`RASQDVGIYVN`	`RATNLAD`	`LEFDEHPLT`	`GFTFSNYWMT`	`ASIDNSGDNTYYPDSVKD`	`GGDITTGFDY`	45	5	10.3	31.0	14	77	4.8
Graft-RAGE	`RASQDVGIYVN`	`RATNLAD`	`LEFDEHPLT`	`GFTFSNYWMT`	`ASIDNSGDNTYYPDSVKD`	`GGDITTGFDY`	45	0	>61.9	ND	0	84	1.0
C7-ABS-RAGE	`RASQSIGSYLN`	`RASSLAS`	`LEFDEHPLT`	`GFTFSSYWMS`	`ASIDQDGSNKYYPDSVKG`	`GGDITTGLDY`	74	0	5.8	17.0	2	85	2.3
DPK9/DP47	`RASQSISSYLN`	`AASSLQS`	`QQSYS–TPLT`	`GFTFSSYAMS`	`SAISGSGGSTYYADSVKG`	n/a	100	n/a	n/a	n/a	n/a	n/a	n/a
Par-A33	`LASEFLFNGVS`	`GASNLES`	`LGGYSGSSGLT`	`GIDFSHYGIS`	`AYIYPNYGSVDYASWVNG`	`DRGYYSGSRGTRLDL`	39	n/a	2.9	2.1	7	74	1.4
Graft-A33	`LASEFLFNGVS`	`GASNLES`	`LGGYSGSSGLT`	`GIDFSHYGIS`	`SYIYPNYGSVDYASWVNG`	`DRGYYSGSRGTRLDL`	39	0	ND	ND	ND	ND	ND
C6-ABS-A33	`RASQFLFNGVS`	`AASNLES`	`QGGYSGSTGLT`	`GFTFSHYGIS`	`SYIYPSYGSTDYASSVKG`	`DRGYYSGSRGTRLDL`	56	0	2.7	2.1	8	74	0.5
DPL16/DP47	`QGDSLRSYYAS`	`GKNNRPS`	`NSRDSSGNHVV`	`GFTFSSYAMS`	`SAISGSGGSTYYADSVKG`	n/a	100	n/a	n/a	n/a	n/a	n/a	n/a
Par-pTau	`SGSD–YDYG-`	`WNDKRPS`	`GAYDGSAGGGI`	`GFTLSSYQMM`	`AGITSRGGVTGYGSAVKG`	`PALDSDQCGFPEAGCIDA`	41	n/a	1.6	0.41	17	70	1.2
Graft-ABS-pTau	`SGSD–YDYG-`	`WNDKRPS`	`GAYDGSAGGGI`	`GFTLSSYQMM`	`AGITSRGGVTGYGSAVKG`	`PALDSDQCGFPEAGCIDA`	41	0	>64.7	NB	90	66	0.8
C21-ABS-pTau	`QGDD–SYYG-`	`GNDNRPS`	`GAYDSSGGGGI`	`GFTLSSYQMM`	`AGITGRGGVTGYADSVKG`	`PALDSDQCGFPEAGCIDA`	65	1	2.1	0.25	3	70	1.4
Com-ABS-pTau	`QGDD–SYYG-`	`GNNNRPS`	`GSYDSSGGHGV`	`GFTFSSYQMS`	`SGITGRGGVTGYADSVKG`	`PALDSDQCGFPEMGCIDA`	76	1	ND	0.50	2	71	0.8

The pTau CDR-L1 is shorter than its DPL16 counterpart by 3 amino acids. Sequence dashes in this CDR are added to show the spacing of sampled residues, based on sequence alignment. Residues differing from human germ line are underlined. CDR definitions used in this manuscript are an expanded definition in comparison with the classical Kabat, including VH positions 26–29 and 49.