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. 2015 Oct 22;34(23):2953–2970. doi: 10.15252/embj.201591437

Figure EV3. EglN2 binds to chromatin and regulates mitochondrial function under hypoxia.

Figure EV3

  • A
    Measurement of oxygen consumption by an oxytherm electrode unit as a function of time for MCF7 cells infected with lentivirus encoding EglN2 shRNA (327 or 328) or control shRNA treated with hypoxia (5% O2).
  • B, C
    Immunoblot from MCF7 (B) or T47D (C) cells infected with lentivirus encoding EglN2 shRNA (326, 327, or 328) or control shRNA (Ctrl) with or without hypoxia (5% O2) treatment.
  • D
    qRT–PCR quantification of mtDNA from MCF7 cells infected with lentivirus encoding HIF1α shRNA (3810), HIF2α shRNA (3804), ARNT shRNA (1770), or control (−) followed by another infection with lentivirus encoding either EglN2 shRNA (326) or control (Ctrl) shRNA with hypoxia (5% O2) treatment.
  • E–G
    Determination of luciferase activity in 293FT cells with plasmids encoding the indicated TETr‐fusion proteins.

Data information: Two‐tailed Student's t‐test was used to examine the P‐values from at least three replicate experiments. Error bars represent SEM. *P < 0.05, ***P < 0.005. NS denotes not significant.