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. 2015 Oct 23;34(23):2971–2983. doi: 10.15252/embj.201592652

Figure EV2. Characterization of self‐renewing and bipotent Oct4‐induced OPCs.

Figure EV2

  • A
    Immunofluorescence images of undifferentiated iOPC‐C2 at passages 5 (P5) and 35 (P35) co‐stained with A2B5 and RIP (left columns), PDGFRα and GFAP (middle columns), and NG2 and Tuj1 (right columns). Cells were counterstained with DAPI. Scale bars, 125 μm.
  • B–E
    The morphological changes of iOPC‐C2 during oligodendrocyte differentiation at days (B) 5, (C, D) 14, and (E) 28. (D) High magnification image of the white square in (C) shows the morphology of oligodendrocytes (green arrowhead) and astrocytes (red arrowhead). Scale bars, 50 μm.
  • F
    Immunocytochemistry analysis of iOPC clones stained with Tuj1 at 14 days after neuron differentiation. Cells were counterstained with DAPI. Scale bars, 125 μm.
  • G
    Immunofluorescence images of iOPC at P35 stained with mature oligodendrocyte markers, GalC (left columns), RIP (middle columns), and MBP (right columns). Scale bars, 75 μm.