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. 2015 Oct 23;34(23):2971–2983. doi: 10.15252/embj.201592652

Figure 3. Global gene expression profiles of Oct4‐induced OPCs.

Figure 3

  • A, B
    Heat map analysis of (A) the global gene expression profiles and (B) 32 OPC and oligodendrocyte lineage enriched genes of Fib, FOct4‐D3, FOct4‐D10, mock, iOPC‐C1, iOPC‐C2, and wtOPCs. The upper color bar codifies the gene expression in the log2 scale. Shown are 767 probes selected based on codified variation ≥ ± 4 across all samples.
  • C
    Pairwise scatter plots of the global gene expression of wtOPCs versus iOPC‐C1/iOPC‐C2, iOPC‐C1 versus iOPC‐C2 and Fib versus iOPC‐C1/iOPC‐C2/wtOPC. The black lines indicate the boundaries of twofold changes in gene expression. The OPC‐enriched genes (Mag, Nkx2.2, Olig1, Olig2, Sox10, Cnp, Cspg4, and Ptprz1) are highlighted with orange circles. Gene expression levels are shown on a log2 scale.
  • D
    Hierarchical clustering of wtOPCs, iOPC‐C1, iOPC‐C2, Fib, FOct4‐D3, and FOct4‐D10.
  • E
    qRT–PCR analysis of mRNA expression level for OPC lineage‐specific genes (Ptprz1, Siat8a, Nkx2.2, Olig1, Olig2, Sox10, Cnp, Mag, and Myrf) in iOPCs relative to fibroblasts. Graphs represent log2‐fold changes after normalization to GAPDH. Data are presented as the means ± SD (n = 3).