Figure 3.

Multiple protein kinase C (PKC) isoforms are expressed and modulated by phorbol 12‐myristate 13‐acetate (PMA) in human podocytes. Human differentiated podocytes were treated with 100 nM PMA for the times indicated (control represents the 0 time‐point) and then Western blot analyses were performed to assess expression levels of the PKC isoenzymes. Immunoreactive bands were then subjected to densitometric analyses and the optical density (OD) values were determined. Numbers indicate OD values compared to those of the control expressions (regarded as 1). For loading control, the expression of β‐actin was determined. These images are representatives from multiple experiments (n = 3 experiments in each case except for PKCα, PKCδ and PKCε, where n = 6, 4 and 4, respectively).