Figure 4.

Protein kinase C (PKC) inhibitors augment the transient receptor potential canonical‐6 (TRPC6)‐mediated Ca²⁺‐response in human podocytes. Single‐cell Ca²⁺‐measurements. OAG (200 μM) was applied as indicated by the bars. (A) Control conditions (no pretreatment). (B–D) Effects of pretreatment with the general PKC blocker GF109203X (1 μM, B), Gö6976, inhibitor of cPKC isoforms (1 μM, C), or Rottlerin, inhibitor of PKCδ (1 μM, D) on the OAG‐induced Ca²⁺‐response. (E and F) Statistical analyses of the above Ca²⁺‐imaging data. Measured peak amplitudes (E) and maximal rate of rise of the transients (F) were plotted as mean ± SEM of multiple independent determinations as indicated above the corresponding columns. *P < 0.05, **P < 0.01, ***P < 0.001 compared to the control group.