Figure 5.

Phorbol 12‐myristate 13‐acetate (PMA) suppresses transient receptor potential canonical‐6 (TRPC6) activity and expression in human podocytes. (A and B) Single‐cell Ca²⁺‐measurements. OAG (200 μM) was applied as indicated by the bars. (A) Control conditions (no pretreatment). (B) Effects of 1 μM PMA‐pretreatment (30 min.) on the OAG induced Ca²⁺‐responses. (C and D) Statistical analysis of the Ca²⁺‐measurement data. Measured peak amplitudes (C) and maximal rate of rise of the transients (D) were plotted as mean ± SEM of multiple independent determinations as indicated above the corresponding columns. *P < 0.05 compared to the control group. (E) Assessment of TRPC6 expression by Western blotting after 100 nM PMA treatment. Immunoreactive bands were subjected to densitometric analyses and the optical density (OD) values were determined. Numbers indicate OD values compared to those of the control expressions (regarded as 1). For loading control, the expression of β‐actin was determined (n = 3 experiments).