Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers for real‐time PCR
| Gene name | Accession no. | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|---|
| TLR4 | NM_019178.1 | GCCGTCTTCAATCTGACTAAT | ACACTGACCACCGATACACT |
| TNF‐α | NM_012675.3 | CCCAATCTGTGTCCTTCTAACT | CACTACTTCAGCGTCTCGTGT |
| IL‐6 | NM_012589.1 | GATTGTATGAACAGCGATGATG | CTCCAGGTAGAAACGGAACTC |
| GAPDH | NM_017008.3 | AACGACCCCTTCATTGACCTC | CCTTGACTGTGCCGTTGAACT |
TLR4, toll‐like receptor 4; TNF, tumour necrosis factor; IL, interleukin.
