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. 2015 Aug 18;19(12):2793–2805. doi: 10.1111/jcmm.12666

Figure 2.

Figure 2

Overexpression of miR‐138 reverses the epithelial‐mesenchymal transition (EMT) of non‐small‐cell lung cancer (NSCLC) cells. 95‐D cells were transfected with miR‐138 mimics or negative control mimics. (A) 48 hrs after transfection, the total proteins were obtained from lysed cells and the expressions of ZO‐1, E‐Cad and Slug were determined by Western blotting. β‐actin was used as a loading control. (B) 24 hrs after transfection, cell migration was evaluated by wound healing assay. (C) 24 hrs after transfection, cell invasion was evaluated using Matrigel‐coated Transwell assay. Results are presentative of three independent experiments and the error bars refer to S.D. **P < 0.01.