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. 2015 Aug 18;19(12):2793–2805. doi: 10.1111/jcmm.12666

Figure 5.

Figure 5

Knockdown of GIT1 inhibits the proliferation of non‐small‐cell lung cancer (NSCLC) cells. (A) Silencing of GIT1 and SEMA4C by siRNAs was determined by Western blotting. 95‐D cells were transfected with three GIT1 siRNAs or two SEMA4C siRNAsrespectively. 48 hrs after transfection, the total proteins were extracted and the expressions of GIT1 and SEMA4C were determined by Western blotting. β‐actin was used as an internal control. (B) Cells proliferation was evaluated by methyl thiazolyl tetrazolium (MTT) assay. (C) Cell cycle was evaluated using propidium iodide staining and flow cytometry. (D) Cells proliferation was evaluated by Ki67 immunostaining assay. Results are presentative of three independent experiments and the error bars refer to S.D. *P < 0.05 and **P < 0.01.