Figure 6.

Ectopic expression of GIT1 rescues miR‐138‐induced cell growth inhibition. (A) Overexpression of GIT1 was determined by Western blot analysis. 95‐D cells were transfected with the GIT1 overexpression plasmids. 48 hrs after transfection, the total proteins were extracted and the expression of GIT1 was determined by Western blotting. β‐actin was used as an internal control. (B) Cells proliferation was evaluated by methyl thiazolyl tetrazolium (MTT) assay. A549 and 95‐D cells were transfected with miR‐138 mimics or control mimics, or co‐transfected with miR‐138 mimics and GIT1 overexpression plasmids. 48 hrs after transfection, cells proliferation was examined. (C) Cell cycle was evaluated using propidium iodide staining and flow cytometry. (D) Cells proliferation was evaluated by Ki67 immunostaining assay. Results are presentative of three independent experiments and the error bars refer to S.D. *P < 0.05 and **P < 0.01.