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. 2015 Aug 18;19(12):2793–2805. doi: 10.1111/jcmm.12666

Figure 7.

Figure 7

MiR‐138 regulates epithelial‐mesenchymal transition (EMT) by targeting GIT1 and SEMA4C. (A) The expressions of ZO‐1, E‐Cad and Slug were determined by Western blotting after the knockdown of SEMA4C or GIT1 in 95‐D cells. β‐actin was used as a loading control. (B) 24 hrs after knockdown of SEMA4C or GIT1 in 95‐D cells, cell migration was evaluated by wound healing assay. (C) 24 hrs after knockdown of SEMA4C or GIT1 in 95‐D cells, cell invasion was evaluated using Matrigel‐coated Transwell assay. (D) The expressions of ZO‐1, E‐Cad and Slug were determined by Western blot analysis. 95‐D cells were transfected with miR‐138 mimics or control mimics, or cotransfected with miR‐138 mimics and GIT1 or SEMA4C overexpression plasmids. Forty‐eight hours after transfection, the expressions of ZO‐1, E‐Cad and Slug were determined by Western blotting. β‐actin was used as an internal control. (E) 24 hrs after transfection, cell migration was evaluated by wound healing assay. (F) 24 hrs after transfection, cell invasion was evaluated using Matrigel‐coated Transwell assay. Results are presentative of three independent experiments and the error bars refer to S.D. *P < 0.05 and **P < 0.01.