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. 2015 Sep 23;19(12):2832–2841. doi: 10.1111/jcmm.12675

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© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Expression of Dickkopf‐related protein 3 (DKK3) in pancreatic cancer cell lines and pancreatic cancer tissues. (A) Expression of DKK3 in pancreatic cancer cell lines detected by Western blot, with β‐actin as a control. (B) Methylation status of DKK3 in pancreatic cancer cell lines. M: methylated; U: unmethylated. (C) Summary of DKK3 expression in human normal pancreas and pancreatic cancer samples. Representative immunostaining of DKK‐3 in human normal pancreas, immunohistochemical (IHC) score 1 (D), 2 (E) and pancreatic cancer samples, IHC score 0 (F), 1 (G) and 2 (H). (I) Overexpression of the DKK3 gene was confirmed by Western blotting in Bxpc‐3 Cells.